Assessing Water Quality with the BD AccuriTM C6 Flow Cytometer White Paper
نویسندگان
چکیده
Flow cytometry can rapidly quantitate bacteria and discriminate them from debris. Researchers at the Swiss Federal Institute of Aquatic Science and Technology (Eawag, Switzerland) have developed a standard flow cytometric staining protocol and a corresponding BD AccuriTM C6 software analysis template to discriminate bacteria from debris in drinking water samples. The template employs a single, fixed gate that contains all bacteria when stained with SYBR® Green I, or only viable bacteria when co-stained with SYBR® Green I and propidium iodide.
منابع مشابه
Using the BD AccuriTM C6 Cytometer for Rapid and Accurate Analysis of the Nuclear DNA Contents of Flowering Plants
The nuclear DNA content (C-value) is a fundamental parameter of eukaryotic cells. Flow cytometry provides the most convenient and accurate way to determine C-values of plants, but sampling of the approximately 480,000 species of angiosperms (flowering plants) remains very limited, only about 1% being reported. For plant species for which C-values are available, these values span an extraordinar...
متن کاملAn Introduction to Window Extension on Digital Flow Cytometers
The optical detectors of a flow cytometer convert all detected light into electronic signals that are subsequently converted into digital data. A sample pulse is the electronic representation of a signal with measurable height, width, and area values. In a BD digital system, the detector output is continuously digitized, and threshold defines the signal level at which the system begins to measu...
متن کاملMice cloned from white adipose tissue-derived cells.
Isolation of different cells from adipose tissue was performed according to the previous reports (Rodeheffer et al., 2008; Sugii et al., 2010). Briefly, the inguinal fat pads of adult male B6D2F1 (8-12 weeks of age) mice were harvested, washed several times with phosphate-buffered saline (PBS) and excised into small pieces. The tissues were digested with 0.1% type I collagenase (Sigma) for 50 m...
متن کاملComparison of a microsphere-based platform with a multiplex flow cytometric assay for determination of circulating cytokines in the mouse
Background Measuring expression profiles of inflammatory biomarkers is important in monitoring the polarization of immune responses; therefore, results should be independent of quantitation methods if they are to be accepted as validated clinical pathology biomarkers. To evaluate effects of differing quantitation methods, the seven major circulating Th1/Th2/Th17 cytokines interleukin 2 (IL-2), ...
متن کاملSimultaneous Fluorescence and Impedance Micro Cytometer – a Modular System
Cytometry is used to identify cells in a variety of fields such as haematology and environmental monitoring. We describe a modular micro-fabricated cytometer that uses on-chip methods to simultaneously excite fluorescence and measure the electrical impedance of individual particles. The integration of impedance and fluorescence cytometry on a single chip provides essentially the same informatio...
متن کامل