Assessing Water Quality with the BD AccuriTM C6 Flow Cytometer White Paper

نویسندگان

  • Erin Gatza
  • Frederik Hammes
  • Emmanuelle Prest
چکیده

Flow cytometry can rapidly quantitate bacteria and discriminate them from debris. Researchers at the Swiss Federal Institute of Aquatic Science and Technology (Eawag, Switzerland) have developed a standard flow cytometric staining protocol and a corresponding BD AccuriTM C6 software analysis template to discriminate bacteria from debris in drinking water samples. The template employs a single, fixed gate that contains all bacteria when stained with SYBR® Green I, or only viable bacteria when co-stained with SYBR® Green I and propidium iodide.

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تاریخ انتشار 2013